the procedure followed in criminology PCR or RFLP method This DNA strands were cut by restriction enzymes into fragments of different lengths. (After denaturation) - These are filled to sort the fragments in a gel (agarose). pnh - In the electrophoresis, pnh the gel appeared at both ends in a buffer pnh solution, which is applied to an electrical voltage. The individual fragments pnh migrate through the electric gel. DNA is negatively charged (due to d phosphate group) & always migrates to the positive pole (anode) There will be a separation of Restriktionsfragemete means of the existing pores in the gel which act like a sieve The separation is based on the electrophoretic mobility (mobility ) Particularly convenient is the property of electrically pnh charged body to use that in an electric field have different migration velocities which the fragments pnh arranged to correspond to its length. Small pieces of DNA migrate faster than large for electrically positive pole; after a few hours are arranged along its length, so all fragments. Between the bases of the DNA prevail pnh attractive forces; one particular base attracts another particular base, two of them form one rung. The finger printer, therefore the gel immersed in a solution that is very short fragments of DNA - contains - so-called probes. These are attracted to the corresponding pnh complementary DNA fragments in the gel. The docked probes visualized by a color reaction; it creates a pattern of thin colored bands - the genetic fingerprint. Evaluation: In the same molecules run in bands through the gel. Several pnh samples can be run parallel to each other at the same time by the same gel. Is the size of some molecules known, can be estimated by comparing the bands with the remaining bands of the size of other molecules. (Comparison sample was selected)
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