Tuesday, July 29, 2014

The purple one square is the gel. Are 2 different chicks per row. Initially, the DNA fragments were


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Yesterday we copied the chosen DNA section quite often. rflp So we can see, because a single DNA segment is incredibly small end up. Now it is so that, for example in us humans the men have two different sex chromosomes. The X and the Y-chromosome. That you might have heard before. In principle, this means that men have two DNA segments for their gender and women only one. In birds, the reverse is true. To look now, how much of the examined chicks and which are male female, the various rflp DNA fragments need to be separated.
Fortunately, the DNA has a charge and can be tightened using an opposite charge. The process by which this is done is called gel electrophoresis. The DNA copies are in a gel. On the other side of the gel is an electrode that attracts the DNA because they are oppositely charged. This is basically like magnets.
The two DNA segments in the female birds vary in size, with the smaller section rflp travels faster through the gel. That you can imagine, so as you would stir with a small spoon through the raw cake mixture, then it goes easier than with a large ladle, since the resistance with the ladle is much greater.
Now that we have now separated the various DNA segments from each other, we need the DNA stain still in order to see them clearly can. This is done with the substance ethidium bromide. So that we can see under UV light, the DNA segments. In the field in which we so see 2 strips 2 DNA segments, the original quill of a female bird has been and where we see only one line, the sample of a male animal has been.
The purple one square is the gel. Are 2 different chicks per row. Initially, the DNA fragments were applied where these dark lines are seen. These are bags, from there, the DNA migrates through the gel. Now you can see for yourself how many males and females rflp are.


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