Smarter ultralow RNA sequencing kit for one cell to the Illumina platform Smarter ultralow RNA Kit

Smarter ultralow RNA sequencing kit for one cell to the Illumina platform Smarter ultralow RNA Kit is a revolutionary means from Clontech to prepare libraries from a single cell (min. 10 pg total RNA) for the analysis of gene expression bial / mutation analysis of new-generation sequencing (NGS) platform Illumina. While digital expression analysis - quantitative sequencing all mRNAs, without any loss of information at its 5 'or 3' end and thus also allows to obtain complete information bial on the mutations present in each transcript. We use proven technology Smarter cDNA synthesis using a specific MMLV RTase and the 5 'and 3' Adaptor produced in a single step, double stranded cDNA, and the resulting cDNA was amplified by PCR LD. Amplification step clogs minimum statistical error and NGS results show a high degree correlating with quantitative real - time PCR. Verification bial of this technology for the measurement of differential gene expression using the example of free circulating cancer cells has been published in the journal Nature Biotechnology (Daniel Ramskold, Vol 30, No. 8, August 2012).