ճ 1 DNA ճ 2 Ի ģ BLAST 㻥 ȶ ܣ Բ Ҫ û ע Ƿ ݿ е GI Ҳ GI ţ Ͳ ճ һ ε ˡ 3 Align Բ ڰ DNA

ÿ һ 4 Ӧ õ ﳤ Ϊ 18 ᡣ ﳤ ȵ Ҫ Ҫ 뷴ӦЧ йء ص Խ ϵ DNA γɹ DNA ø ϵ ȶ ģ ԽС
Ҫ Ƚ ȵ 5 棬 ʵ ȣ ʹ PCR ӣ ô ʵ ȣ DNA ϡ һ 4 桫 6 治 Ӱ PCR ʣ һ һ ģ 55 桫 75 仯
ѡ Ƭ ʱ ñ ģ Ķ ṹ йؼ Ԥ Ŀ Ƭ ε ȶ ṹ ѡ ģ 塣ʵ ܣ G С 58.6lkJ/mol ʱ ɹ һ ʱ 7-deaza-2’- GTP ȡ dGTP ijɹ а ġ
ճ 1 DNA ճ 2 Ի ģ BLAST 㻥 ȶ ܣ Բ Ҫ û ע Ƿ ݿ е GI Ҳ GI ţ Ͳ ճ һ ε ˡ 3 Align Բ ڰ DNA Ƿ ж Դλ ˡ
3’ 쿪ʼ ĵط Ҫ ʹ ʼ 3’ Ӧ 3 G C ʹ G+C 3 ′ Ҳ γ κζ ṹ ܣ PCR AS-PCR Ӧ У 3 ′ 䡣 3 ′ Ҫ ӵĵ 3 λ ӵĵ 3 λ Ӱ Ч ʡ
?Ӧ ڻ У ɷ ṹ ṹ ռ λ Ӱ ģ ĸ Խ ϡ жϣ 3bp 䲻Ӧ ô ڻ ԣ Ӧ 3 ′ Ļ ص Է γɡ һ һ 䲻Ӧ 4 Դ Ի ԡ
5’ Ӱ 첻 ˣ Ա ζ Ӱ ԡ 5 ′ ΰ ø λ 㣻 ء ӫ ظ Eu3+ ȣ 뵰 ʽ DNA У ͻ λ 㡢 ȱʧͻ к һ еȡ ļ ٻ Ӱ Ч ʣ Ӵ γɵļ ʣ Ϊ һ IJ Ҫ ʵ “ ”
ø λ ǽ PCR ǿ ʹ õ Ρ һ ø λ ø λ 5’ Ҫ 2 3 Dz ø Ҫ ı Ŀ Dz ģ 磺 Sal Ҫ EcoR Ҫ 1 Not Ҫ 2 Hind 3 У ʱ һЩС ɣ ҿ Բο ʵ ǰ ķ һЩ б ﶼ õġ
һ ڽ PCR Ӧ ʱ ø У Ҫע һЩ ø PCR Ӧ е ø з Ӧ ʣ Ȼ 㵫 Ƽ ʱ ǰ PCR պ ø Ч 룬 л ʹ ø Ӹ ӡ һ 鷳
LIC ȫ Ligation-Independent cloning microarray Navogen ר Ϊ 䲿 pET һ LIC Ʊ pET в 12–15 microarray ճ ˣ Ŀ IJ Ƭ Ӧճ Ŀ IJ Ƭ ε 5' Ҫ LIC 廥 T4 DNA ۺ ø 3' → 5' л Ծ ʱ 伴 ڲ Ƭ γɵ ճ ˡ Ʊ õIJ Ƭ κ 廥 γɲ dz ٸ Ч Ϊ
T ճ ʱ Ҷ ޣ Ƿ 㣬 ĸ С ô ġ Ǻ Ƿ TA Ƭ ζ У Ժ Invitrogen Ƴ Զ 壬 һ ĸ ͻ ļ GTGG PCR ʱҲҪ Ӧ ļ У Ƭ ξ “ з ” ˡ
Clontech BD ʱ Ƴ ģ 2004 ʵ һ ѣ ѡ ȴ Լ ͶƱΪ һ ӭ Լ 䲽 Ǽ ģ ø 賤ʱ ķ Ӧ Ҫ ʱ һ Ի У Ȼ PCR Ի 뵽 BSA In-Fusion ø Һ У ð Сʱ Խ ת ˡ ر ʺϴ ת
Ҫ ļ ǻ ٻ Ӱ 쵽 PCR Ӧ ڼ Not ø λ Ⱦͻ ʶ 8 ȫΪ GC ʹ һ Ʊ ʮ ѣ Ϊһ Զ ᵽ ʵ Ӧ ϡ Щ ʱ “ʵ Ǽ Ψһ ” һ Ҫ һ Բ е ˡ л ƣ õ http://blog.sciencenet.cn/blog-350702-342275.html   Կ ѧ Ө ת ע һƪ [ת ][ת]64bit Linux װMatlab.R2009b.Unix.ISO-TBE һƪ [ת ] mkTextEncoding: invalid microarray argument Ľ
ȫ ߵ • [ת ] ת Linux º Matlab • Debug while installing DNAA on Ubuntu system • microarray [ת ] mkTextEncoding: invalid argument Ľ • [ת ][ת]64bit Linux װMatlab.R2009b.Unix.ISO-TBE