һ ij Ϊ15-30 dibb bp õij Ϊ18-21bp ʣ Ϊ ᵼ ȴ 74 棬 Taq DNA ۺ ø з Ӧ ȴ 24 յ ﲢ ζ Ÿ ߵ ԣ ұȶ ӽ Ӷ һ ﳤ

һ ij Ϊ15-30 dibb bp õij Ϊ18-21bp ʣ Ϊ ᵼ ȴ 74 棬 Taq DNA ۺ ø з Ӧ ȴ 24 յ ﲢ ζ Ÿ ߵ ԣ ұȶ ӽ Ӷ һ ﳤ ȴ 16bp DZ Ҫ ģ 18bp һ  
һ Ҫ 55 -65 档 Ӧ Tm һ һ 2 ڡ С 5 һ 㲻 Ӱ PCR IJ ʣ һ һ ģ 55 桫 80 ӽ 72 Ϊ Tm Tm һ Ӧ 20 ڡ   dibb
§ 壨 䲻Ӧ 4 Դ Ի • dibb 3 ’ н нṹ 3bp Ҫ 3 ’ γɷ нṹ Ӱ DNA ۺ ø 졣 ṹ Բ 4.5 Ϊ ã м 5 ’ ʵ ſ  
ȥ Ϊ 3 ’ Ӧ ν ģ ϲ Ч 죬 3 ’ Ϊ G C ڵĹ Ϊ 5 ’ Ӧ ȶ ṹ 3’ ڼ Ե Ϊ ȶ Խṹ 3 ’ ѡ A T A G C һ ýϵ Tm -5 Ϊ PCR ȡ GC Ҳ
ģ 岻 3 ’ һ Ϊ T G C ѡ A ϱ ĩλΪ T ʱ ʹ ڴ Ҳ ĺϳɣ ĩλΪ A ʱ ʱ G C У ɼ ģ ʱѡ A õ http://blog.sciencenet.cn/blog-442719-358854.html   dibb Կ ѧ ı ת ע һƪ [ת ] й һƪ [ת ]Ⱦɫ 岽 (chromosome walking)