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Answers Forum overview pharmaceuticals -> Other Author Message akva Joined: 15/05/2011 Beitrge: 1 Posted on 15 May 2011 13:22 Title: SDS-PAGE, gel electrophoresis
My question: What is the difference between SDS-PAGE and gel electrophoresis? My ideas: No idea =) but I am grateful for an answer. Akvar Guest Posted 15 May 2011 13:28 Title: resp. gel electrophoresis in polyacrylamide gel? magician4 Moderator Joined: 05/10/2009 Beitrge: 8334 Location: pharmaceuticals Hamburg Posted 15 May 2011 17:47 Title: the difference is that due to the added SDS to the polyacrylamide (otherwise the procedure is identical), the running property of proteins pharmaceuticals is significantly manipulated in the e-field, since they are virtually always held "puffer-like" anionic. thus resulting in a opposite normal "electrophoresis on polyamide gel" vera modified separating image, and you can for example detect sudden things pharmaceuticals that were previously stored about the proteins o.ae.. http://de.wikipedia.org/wiki/SDS-PAGE pharmaceuticals greeting ingo _________________ saves a month in the laboratory a smooth but ne quarter of an hour at the library!
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