The copper color did not work with us. This could be due to the fact that we wanted to color with c

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Same time off the front: When working the four of you should before decide genbank exactly who, what, how and when does. Especially with the dilution series, it is very confusing when everyone wants to help somehow and one must constantly Pipette different tiny amounts. In short, consultation is all here.
Likewise, if you pipetted together the electrophoresis gels. Water, TRIS and SDS can be summed tip in advance. But as soon as TEMED was added it should go quickly. As added last nor APS, mix well and pipette into the caster. After three quarters of an hour, the gel is polymerized.
During assembly genbank of the chamber must always be taken, that's all tight. It's really annoying when you are pipetted into the gel casting stand and the bottom of the whole shit runs back out and makes a mess ... Also there is no one then a gel ^ ^ Otherwise, the gel should be seen rather as unstressiges internship.
We tried 4 Färbemethden: Coomassie, silver, copper and specific AK-staining. genbank The Coomassie staining clearly works best and without complications. There was a really herzeigbares gel. The milk proteins α-lactalbumin (approx. 14 kDa), β-lactoglobulin (approx. 17 kDa) could be unambiguously assigned. Other bands in the range of 26, 30 and 34 kDa still show some of the caseins. The concentration dependence was very nice to be recognized. The soy proteins were indeed separated also concentration-dependent, but could not be assigned genbank to them, since it was only around fragments of glycinin and β-Conglycinins. Both have relatively large masses (162-300 kDa) protein markers spanned only our 10-170 kDa.
Silver staining showed clearly less gangs. Since the silver ions to react with glutamine, asparagine and cysteine, the bands were also do not contain these AS is not displayed. Oh yes, unfortunately, the proteins are broken after this staining and may not be used.
The copper color did not work with us. This could be due to the fact that we wanted to color with copper sulfate. The references give as a staining reagent but copper chloride at ...
Wherein AK staining α-tubulin was electrophoretically separated and blotted onto a nitrocellulose membrane. Here you have to work very carefully. genbank THe diaphragm genbank must be handled only with forceps genbank and she is very very fragile. So work slowly!
You can use the Ponceau S staining around the semydry Western blot to verify. It is better you look obs worked before because of expensive antibodies drangeht ^ ^ The advantage of Ponceau S, that it interferes with the net later following AK-proof and can be washed out again. Ponceau S is in the range of 55 kDa (= weight of tubulin) beautiful spots. Juhu blotting worked. Since α-tubulin-specific antibodies were used in this method is a specific staining method. The Coomassie, buyers and silver genbank stains are nonspecific staining.
All in all, the gel electrophoresis genbank to separate a versatile method for proteins and nucleic acids and to analyze. The evaluation genbank is relatively genbank easy to perform. genbank There are also beautiful computer programs, a Around the heights of the marker on the gangs.
Do I need to check times. In the editor everything siehts einheitlch from? But you're right on