Friday, May 1, 2015

Original language English Qualification Doctor of Philosophy Supervisor (s) / World of Elsas, Jan D

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The soil is a very heterogeneous and dynamic system for the micro-organisms living in it. The biological diversity of the living soil is very high, and estimates of the numbers rt pcr of types of bacteria per gram of soil, amount to up to a million. These microorganisms may react strongly to, and fluctuate with certain environmental factors (disturbances), as abiotic factors temperature, pH, moisture content and structure or texture of the soil as well as biotic factors composition and diversity of the microbial community present. Said fluctuations may affect the dynamics and activity of soil organisms and their interactions. This can, in summary, the determination of normal "operating range" (NOR) of the bottom. Normality is defined here as including the normal fluctuations in function in response rt pcr to all the conditions that may occur in the soil, through natural or (regular) path anthropogenic. A consideration of the biogeochemical cycles of the soil showed that the nitrogen cycle is strongly influenced. Both symbiotic nitrogen fixation as nitrification have been proposed as typical processes that are sensitive to disturbances. These processes might therefore be used as indicators of good stress conditions in the soil. In light of these arguments in this thesis the abundance, structure (community structure) and function of microorganisms that are involved in certain nitrogen cycle processes followed in a series rt pcr of selected Dutch soils. Chapters 1 and 2 introduce the subject in a theoretical sense. In chapters 3 and 5 are the fluctuations described in the structures (compositions) of the nitrogen-fixing micro-organisms. In these analyzes, it was found that different types of soil could be distinguished on the basis of these structures. In particular microbiota was studied in soils with higher clay content (clay soils) more diverse and also the amplitudes rt pcr of the fluctuations in time were higher than those in soils with lower clay content (sandy rt pcr soils). In addition, factors such as soil were the content of ammonium, pH and texture strongly correlated with the variations in the size, diversity and structure of the nitrogen-binding microbial communities. This indicated that fix nitrogen are sensitive to the abiotic parameters of the soil, which makes them suitable as possible indicators of disturbances in the soil. In Chapters 4 and 6 studied the dynamics of the ammonium oxidizers, as well as the effects of abiotic factors in the soil on the size, structure and diversity of these microbial communities. The data obtained showed that soil pH and soil type were major factors affecting the size and structure of the archaeal (AOA) and bacterial (AOB) ammonium oxidizers, in addition to their function, determine. This data can serve as the basis on which the NOR for nitrification in (agricultural) rt pcr soils can be determined. Because the parameters, type of soil and soil pH are intrinsically linked to each other, is set up in chapter 7 a microcosm experiment in which the influences rt pcr of the soil texture and soil pH on the abundance and function of both fix nitrogen as ammonium oxidizers have been determined separately. The results indicated that the AOA are involved in nitrification in soils that have recently been disrupted, while AOB more relevant might be under undisturbed soils. Furthermore, the results showed that the higher abundance of nitrogen cycle micro-organisms in clayey soils are likely to relate to the higher pH in these soils, and not to the texture type. Finally, in chapter 9, a mathematical model describing a quantitative approach of the NOR function of soil proposed. This modeling approach will be important for future provisions on the quality of soils.
Original language English Qualification Doctor of Philosophy Supervisor (s) / World of Elsas, Jan Dirk, Supervisor Falcao Salles, Joana, Co-Supervisor Publication d

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