Results: The serum specimens from 31 people team were tested for IgM and IgG antibody against parvo

Eve Nowalany-Kozielska 1, Monika Kozieł 1, Dorota Kwiatkowska Domal-2, Simon Dworniczak 3, Romuald Wojnicz 4, Celina Shabbir 1, Wojciech Jacheć 1, Damian Kawecki 1, Slawomir karyotype Smolik 2, Ludmila Węglarz 2, George Kozielski 3
1 II Department of Cardiology, Faculty of Medicine, Division of Medical Dentistry in Zabrze, Silesian Medical School, 2 Department of Biochemistry, Faculty of Pharmacy karyotype and Laboratory Medicine in Sosnowiec 3 Department of Pulmonary Diseases and Tuberculosis, Faculty of Medicine and Division of Medical Dentistry in Zabrze, Silesian Medical School, 4 Department and Department of Histology and Embryology, Faculty of Medicine and Division of Medical karyotype Dentistry in Zabrze, Silesian Medical School ABSTRACT:
Introduction: In connection with the increase in the number of documented cases of myocarditis diagnosis of viral etiology of this disease is one of the greatest challenges of modern cardiology. The purpose of this retrospective analysis was to determine the usefulness of viral serological tests in patients with non-ischemic systolic heart failure and confirmed by PCR test the presence of parvovirus B19 (PVB-19) in myocardial biopsies.
Results: On the basis of serological virological antibodies against PVB19 IgM was found in 3 patients, and IgG - in 23 patients. In 5 of these 26 patients demonstrated the presence of endomyocardial clippings PVB-19 genome, therefore, in 5 patients antibodies karyotype PVB-19 being detected coexisted with endomyocardial biopsy. The presence of PVB-19 genome in endomyocardial biopsy does not correlate with the designated serologically as IgM and IgG antibodies against this virus.
Conclusions: Serological tests are not useful in the diagnosis of patients with non-ischemic systolic heart failure and PVB-19 infection. The results of serological tests fall more often positive than the presence of the virus in specimens endomiokardialnych. KEY WORDS: parvovirus B19 myocarditis, endomyocardial biopsy, serological tests
Results: The serum specimens from 31 people team were tested for IgM and IgG antibody against parvovirus B19. IgM antibodies were identifi karyotype ed in 3 people team and IgG antibodies karyotype were identifi ed in 23 patients. All of the people team underwent endomyocardial biopsy revealed chronic Which active myocarditis in 10 people team (32.4%), chronic persistent myocarditis in 14 people team (45.1%) and no myocarditis in 7 people team (22.5%).
Conclusions: Virus serology has no relevance for the diagnosis of non-ischemic systolic heart failure Caused by parvovirus B19 infection. The result of serological tests are positive more frequently than the biopsy specimens results. KEY WORDS: parvovirus B19 myocarditis, endomyocardial BIOSes, virus serology
Myocarditis INTRODUCTION (ZMS) is a disease of various etiologies, most often caused karyotype by cardiotropic viruses properties, especially Coxsackie B, adenovirus, and parvovirus B19 (PVB-19) [1]. Typically sharp ZMS resolves spontaneously and completely, occasionally karyotype can pass in chronic phase, manifesting clinically as dilated cardiomyopathy and heart failure. The inflammatory process involves cardiomyocytes, interstitial tissue, vessels, and sometimes the pericardium [2, 3]. Diagnosis of viral ZMS is one of the greatest challenges of modern cardiology. The diagnosis should be confirmed ZMS stretch of the myocardium by endomyocardial biopsy (EMB), which is considered the "gold standard" according to the current diagnostic criteria. Complete diagnostics of viral origin ZMS should include an evaluation of histopathological, immunohistochemical and molecular karyotype study aimed to demonstrate the presence of genetic material of the virus in specimens cardiotropic endomiokardialnych [4, 5]. The use of endomyocardial biopsy in clinical practice limits the availability of methods and experience in assessing karyotype the results of the study stretches the heart muscle. Serological karyotype tests are often virological and willingly used in the diagnosis of myocarditis of viral etiology, despite the lack of convincing evidence from clinical trials about their skuteczności.Celem karyotype of this retrospective analysis was to determine the diagnostic value of viral serological tests in patients with non-ischemic systolic heart failure presence of the PVB-19 in the myocardium.
MATERIAL AND METHODS: karyotype The study included 31 patients with systolic heart failure of non-ischemic myocardium diagnosed in 2001-2006 in the Second Department and the Department of Clinical Cardiology, Zabrze, where he underwent routine non-invasive cardiology, including echocardiography study in the rest cznemu and invasive karyotype - Coronary angiography ii endomyocardial biopsy. Clippings endomiokardialnepobrano under the "Diseases of the heart muscle." All diagnostic and therapeutic procedures were subject to the consent of the Bio