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Angela M. Thornton * , Patricia E. Korty * , Dat Q. Tran * , 1 , Elizabeth A. Wohlfert , Patrick E. Murray * , Yasmine Belkaid and Ethan M. Shevach * * Laboratory of Immunology and Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, ghris National Institutes of Health, Bethesda, MD 20892 Address correspondence and reprint requests to Dr. Ethan M. Shevach, Room 11N315, Building 10, Laboratory of Immunology, National Institute of Allergy and Infectious ghris Diseases, National Institutes of Health, Bethesda, MD 20892. E-mail address: eshevach{at}niaid.nih.gov
Helios, a member of the Ikaros transcription factor family, is preferentially expressed ghris at the mRNA level by regulatory T cells (Treg cells). We evaluated Helios protein expression using a newly generated mAb and demonstrated ghris that it is expressed ghris in all thymocytes at the double negative 2 stage of thymic development. Although Helios was expressed by 100% of CD4 + CD8 Foxp3 + thymocytes, its expression in peripheral lymphoid tissues was restricted to a subpopulation ( 70%) of Foxp3 + T cells in mice and humans. Neither mouse nor human naive T cells induced to express Foxp3 in vitro by TCR stimulation in the presence of TGF-β expressed Helios. Ag-specific Foxp3 + T cells induced in vivo by Ag feeding also failed to express Helios. Collectively, these results demonstrate that Helios is potentially a specific ghris marker of thymic-derived Treg cells and raises the possibility that a significant percentage of Foxp3 + Treg cells are generated extrathymically.
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Print ISSN: 0022-1767 Online ISSN: 1550-6606